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Amino Acids and Peptides

簡要描述:The column is suitable for the separation of derivatives of amino acids (A) and peptides (P). The common procedures are summarized in the following table. If samples are not often analyzed, esp. DABSY

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詳細(xì)介紹

Amino Acid and Peptide Analysis

 

UltraSep ESD AP

  

The column is suitable for the separation of derivatives of amino acids (A) and peptides (P). The common procedures are summarized in the following table. If samples are not often analyzed, esp. DABSYLchloride shows advantages.

Comparison of different procedures for derivatization

Procedure

Detection

Limit of detection

Remarks

DABS

Absorption
436 nm

< 1 pmol

Stable derivatives; primary and secondary amino acids can be determined

OPA

Fluorescence
330/440 nm

< 1 pmol

Pro, Hypro and Cys cannot be determined 

FMOC

Fluorescence
265/315 nm

< 1 pmol

His and Cys will be detected only in part

DABS = DABSYLchloride (Dimethylaminoazobenzenesulfonylchloride)
OPA = o-Phthalaldehyde
FMOC = 9-Fluorenylmethoxycarbonylchloride

 

Examples of Analyzed Compounds

Analyzed in Application

Go to

 

Alanine (Ala)

AA

 

 

Arginine (Arg)

AA

AA

 

Aspartic acid (Asp)

AA

 

 

Cystine (Cys2)

AA

EPHARM6

 

Glutamic acid (Glu)

AA

 

 

Glycine (Gly)

AA

 

 

Glutathione

EPHARM6

 
 

Histidine (His)

AA

 

 

Isoleucine (Ile)

AA

 

 

Leucine (Leu)

AA

 

 

Lysine (Lys)

AA

 

 

Methionine (Met)

AA

 

 

Ornithine (Orn)

AA

 

 

Phenylalanine (Phe)

AA

 

 

Proline (Pro)

AA

 

 

Serine (Ser)

AA

 

 

Threonine (Thr)

AA

 

 

Tyrosine (Tyr)

AA

 

 

Valine (Val)

AA

 

 

COLUMNS and  PRICES  ()
 Column size (mm)
250 x 4250 x 3250 x 2125 x 4125 x 3125 x 2
UltraSep ESD AP360.00340.00355.00320.00295.00290.00
 

Size (mm)

 
10 x 410 x 310 x 2
Precolumn cartridges
(Price per pack = 5 pieces)
170.00170.00170.00

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